Ho chromatography e sebetsang hantle haholo ea metsi (HPLC), karolo e 'ngoe le e 'ngoe e bapala karolo ea bohlokoa ho fihlelleng liphetho tse nepahetseng le tse tšepahalang. Har'a likarolo tsena, li-tubing tsa HPLC li ka 'na tsa bonahala eka ke tsa bobeli, empa ha e le hantle li bohlokoa bakeng sa ho netefatsa botsitso le ho nepahala ho hlokahalang li-lab tsa lipatlisiso. Ho utloisisa hore na hobaneng li-tubing tsa HPLC li le bohlokoa le hore na u ka khetha e nepahetseng joang ho ka etsa phapang e kholo ho boleng ba liphetho tsa lab ea hau.
Karolo ea HPLC Tubing ho Labs ea Lipatlisiso
HPLC e sebetsa ka li-tubingjoalo ka tsela ea hore sampole ea mokelikeli le solvent e tsamaee ka har'a sistimi ea HPLC. Le liphapang tse nyane tsa li-tubing li ka ama sekhahla sa phallo, khatello le boleng ba karohano. Bakeng sa bafuputsi ba ikemiselitseng ho hlahisa liphetho, ho khetha li-tubing tse nepahetseng ke senotlolo. Ka ts'ebeliso ea litlhare, tlhahlobo ea tikoloho, le biochemistry, khetho ea HPLC tubing e ama ka kotloloho ho nepahala ha lithuto tse fapaneng.
1. Litaba tsa Boitsebiso: Ho Khetha Tubing e Nepahetseng
Thepa ea HPLC tubing e ama ts'ebetso haholo. Stainless steel, PEEK (polyether ether ketone), le silica e kopantsoeng ke lisebelisoa tse tloaelehileng, e 'ngoe le e' ngoe e loketse mefuta e itseng ea litlhahlobo. Mohlala, li-tubing tsa tšepe tse sa hloekang li tšoarella nako e telele 'me li hanela khatello e phahameng, e leng se etsang hore e be sebaka se loketseng tlhahlobo ea boleng bo holimo. PEEK, ka lehlakoreng le leng, ha e sebetse ka lik'hemik'hale ebile ha e na tšepe, e etsa hore e tšoanelehe bakeng sa lits'ebetso tsa likokoana-hloko moo li-ion tsa tšepe li ka sitisang metsoako e bonolo.
Thuto-pale: Stainless Steel vs. PEEK Tubing
Phuputsong e mabapi le metsoako ea meriana, lab e fumane hore li-tubing tsa tšepe tse sa hloekang li fana ka nako e telele empa li ile tsa ama bahlahlobisisi ba itseng hanyenyane. Ho fetohela ho li-tubing tsa PEEK ho felisitse taba ena, ho bonts'a bohlokoa ba khetho ea thepa ho boloka sampole ea botšepehi.
2. Diameter e ka Hare le Tšusumetso ea Eona ho Phallo
Bophara ba ka hare ba li-tubing tsa HPLC ke ntlha e 'ngoe ea bohlokoa. Sebaka se senyenyane sa ka hare se ka thusa ho finyella kutlo e phahameng ka ho fokotsa ho atolosoa ha lihlopha, empa hape e hloka taolo e nepahetseng haholoanyane ea khatello. Ka lehlakoreng le leng, bophara bo boholo hangata bo loketse litekanyetso tsa phallo e potlakileng empa bo ka fokotsa tharollo. Ho khetha li-tubing tse nang le bophara bo loketseng ho bohlokoa ho leka-lekanya kutloisiso le sekhahla sa phallo le litlhoko tsa khatello.
Ntlafatsa Tubing bakeng sa Analytical kapa Preparative HPLC
Bakeng sa tlhahlobo ea HPLC, bophara bo bonyenyane ba ka hare (mohlala, 0.13 mm) hangata bo fana ka karohano e ntle. Ka lehlakoreng le leng, HPLC e itokisang, e sebetsanang le mehlala e meholoanyane, hangata e rua molemo ho tloha bophara bo boholo ho tšehetsa phallo e potlakileng le ho fokotsa khatello ea morao.
3. Bolelele le Khatello: Ho Fumana Tekano e Nepahetseng
Bolelele ba li-tubing tsa HPLC bo ama tsela ea phallo le khatello e akaretsang ea sistimi. Li-tubing tse telele li ka lebisa khatellong e eketsehileng, e ka 'nang ea hloka hore ho lokisoe litlhophiso tsa pompo. Sena se bohlokoa haholo lits'ebetsong tsa khatello e phahameng joalo ka gradient HPLC, moo bolelele ba li-tubing bo amang ka kotloloho nako ea ho boloka le boleng ba karohano. Ho boloka li-tubing li le khuts'oane ka hohle kamoo ho ka khonehang ntle le ho sekisetsa lintlha tsa khokahano ho ka thusa ho fihlela tekanyo e nepahetseng ea khatello.
Khutsufatsa Tubing ho Fokotsa Khatello ea Sisteme
Lits'ebetsong tsa khatello e phahameng, ho fokotsa bolelele ba li-tubing ho ka fokotsa khatello ea khatello, ho matlafatsa botsitso ba sistimi le ho boloka bophelo ba pompo. Li-Labs tse etsang tlhahlobo ea tšebetso e phahameng li tlalehile phokotso e hlokomelehang ea litlhoko tsa tlhokomelo ka ho ntlafatsa bolelele ba li-tubing.
4. Ho lumellana le Lik'hemik'hale le Li-solvents
Ho tsamaellana ha li-tubing tsa HPLC le lik'hemik'hale tse fapaneng le lihlapolli ho bohlokoa, haholo lilabong tse sebetsanang le mefuta e mengata ea metsoako. Li-solvents tse ling li ka senya lisebelisoa tsa li-tubing ha nako e ntse e ea, tse lebisang tšilafalong kapa ho lutla. Pele u khetha li-tubing, etsa bonnete ba hore lia lumellana le lihlapolli tse sebelisoang hangata ka laboratoring ea hau ho thibela litaba tsena.
Mohlala oa Bophelo ba 'Nete: Ho lumellana ho Labs ea Tlhahlobo ea Tikoloho
Lab ea tlhahlobo ea tikoloho e etsang tlhahlobo ea chefo e bolaeang likokonyana e fumane hore thepa ea eona ea liphaephe e ne e sa lumellane le litlhare tse itseng tse sebelisoang tlhahlobong, e leng se bakang hore ho nkeloe sebaka khafetsa. Ho fetohela ho li-tubing tse tsamaellanang le lik'hemik'hale ho fokolitse tlhokomelo haholo le ts'epahalo e ntlafalitsoeng ea sephetho.
5. Ho etsa bonnete ba hore Li-tubing li Hloekile ebile ha Li na Tšilafalo
Tšoaetso e ka senya liphetho tsa HPLC habonolo, 'me li-tubing e ka ba mohloli o patiloeng oa taba ena. Ho hloekisa kamehla le ho nkela li-tubing sebaka khafetsa ho thusa ho boloka bots'epehi ba sistimi ea HPLC. Li-laboratory tse ngata li kenyelletsa tlhokomelo e reriloeng 'me li fetola li-tubing nako le nako ho qoba likotsi tsa tšoaetso, haholo-holo mafapheng a maemo a holimo joalo ka lipatlisiso tsa meriana le biochemical.
Theha Mokhoa oa Tlhokomelo ea Li-Tubing
Ho kenyelletsa tlhahlobo ea khafetsa le liprothokholo tsa ho hloekisa bakeng sa li-tubing tsa HPLC ho ka thibela ho bokellana ha masala le tšilafalo, e leng se lebisang ho liphetho tse tsitsitseng haholoanyane. Li-labs tse ling li sebelisa li-solvents flushing kapa li-cycle tse khethiloeng tsa ho hloekisa ho netefatsa hore li-tubing li lula li se na masala.
Khetho e nepahetseng ea li-tubing tsa HPLC e ka ntlafatsa haholo ts'ebetso le ho nepahala ha li-lab tsa lipatlisiso. Ho tloha ho khetha thepa e nepahetseng le bophara ba ho laola khatello le ho netefatsa ho lumellana ha lik'hemik'hale, mohopolo o mong le o mong o ama katleho ea tlhahlobo ea HPLC. Ka ho ela hloko lintlha tsena ka hloko, bafuputsi ba ka fumana liphetho tse tšepahalang, tse ka hlahisoang hape tse ntšetsang lithuto tsa bona pele le ho kenya letsoho tsoelopeleng e batsi ea saense. Tlhokomelo e nepahetseng ea li-tubing le khetho ha e tšehetse ts'ebetso ea lab feela empa hape e sireletsa boleng ba liphetho tsa lipatlisiso, ho etsa hore HPLC e be karolo ea bohlokoa maemong afe kapa afe a laboratoring.
Nako ea poso: Oct-30-2024