Ka ho ts'oaroa ha chromatography e phahameng (HPTC), karolo e 'ngoe le e' ngoe e bapala karolo ea bohlokoa ho fihlela liphello tse nepahetseng le tse tšepahalang. Har'a likarolo tsena, ho ka utloahala e le sa bohlokoa, empa ho hlile ho bohlokoa bakeng sa ho netefatsa maemo le ho nepahala ha le langoana la lipatlisiso. Ho utloisisa lebaka leo Hplc tubing e bohlokoa ebile mokhoa oa ho khetha e nepahetseng e ka etsa phapang e nepahetseng ea liphetho tsa sephetho sa Lab.
Karolo ea HPLC e ntse e le Labs ea lipatlisiso
Likhetho tsa HPTCjoalo ka tsela ea kemiso ea mokelikeli 'me u lebelle ho tsamaea ka sistimi ea HPLC. Le mefuta e fapaneng e nyane ka tubing e ka ama litheko, khatello le boleng ba karohano. Bakeng sa bafuputsi ba sepheo bakeng sa litholoana tse khothatsang, ho khetha tubi e loketseng ke senotlolo. Ka likopo tsa meriana, tlhahlobo ea tikoloho le biochemist, khetho ea liphuputso li tiisa ka kotloloho ho fana ka lithuto tse fapaneng.
1. Litaba tsa lintho tse bonahalang: Ho khetha likhoele tse nepahetseng
Boitsebiso ba Hplc li ama ts'ebetso e ngata haholo. Letlapa le nang le tšepe, Peethether Ether (Polythether Ether Ka mohlala, tubi ea tšepe e nang le letsoai le sa tšoaneleheng ebile e hanyetse khatello e phahameng, e e etsa hore e be le tlhahlobo ea baeti e phahameng. Peek, ka lehlakoreng le leng, ho na le mamati a lik'hemik'hale ebile a se na maoatle, a etsang hore e loketse lits'ebetso tsa tlhaho moo ions ea tšepe e ka kena-kenana le metsoako e metle.
Thutong ea linyeoe: steel e se nang mabele vs. Peek Tiking
Thutong ea metsoako ea metsoako, lab e fumane hore masholu a tšepe a nang le spain a fane ka litlhahlobo tse phahameng empa hanyane hanyane. Ho fetohela ho Peek Tike Ho ile ha Tlisetsa Bohlokoa ba ho Fumana Boitsebiso ba boitsebiso ba ho boloka botšepehi ba mohlala.
2. Bophara ba ka hare le phello ea eona ho phallo
Sesebelisoa sa kahare sa Hplc tubing ke ntho e 'ngoe e tebileng. Bophahamo bo nyane bo ka hare bo ka thusa ho fihlela kutloisiso e phahameng ka ho fokotsa sehlopha se phallang ba sehlopha, empa e boetse e hloka taolo e nepahetseng haholoanyane. Ka lehlakoreng le leng, bophara bo boholo hangata bo loketse litheko tse potlakileng tsa ho phalla ka potlako empa li ka fokotsa qeto. Ho khetha marang-rang le tšollo e nepahetseng ho bohlokoa ho lekanya maikutlo a lekanyelitsoeng ka litekanyetso tsa phallo le litlhokahalo tsa khatello.
Fokotsa likhohlano bakeng sa tlhahlobo ea tlhahlobo kapa ho lokisoa ha hiptc
Bakeng sa li-hiplc, bophara bo nyane ba kahare (mohlala, 0.13 mm) hangata e fana ka karohano e ntle. Ka lehlakoreng le leng, li-Iplc tse sebetsang, tseo hangata li tsamaisang lipalo tse kholo, hangata melemo ea mohlala, hangata melemo ea mohlala, hangata melemo ea mohlala, hangata melemo ea mohlala, hangata melemo e meputso e kholo ho tšehetsa ho phalla ka potlako le ho fokotsa morao.
3. Bolelele le khatello: Ho fumana chelete e nepahetseng
Bolelele ba Hplc bo ama tsela e phallang le khatello ea tsamaiso ea tsamaiso. Mafutsana a telele ho ka baka khatello e eketsehang, e ka hlokang liphetoho tsa pompa. Sena ke sa bohlokoa haholo likopo tsa khatello ea khatello e phahameng joaloka hplc e le Gratient Ho boloka manonyeletso ka bokhutšoanyane ka moo ho ka khonehang ntle le ho tlohela lintlha tsa khokahano ho ka thusa ho fihlela tekanyetso e ntle ea khatello.
Khutsufatsa ho fokotsa khatello ea sistimi
Ka lits'ebetso tsa khatello ea khatello ea khatello, bolelele ba khatello bo ka fokotsa ho aha ha khatello, ho ntlafatsa khatello ea khatello ea khatello, ho ntlafatsa botsitso ba tsamaiso ea pompo. Li-labs tse tsamaisang tlhahlobo ea lintho tse ngata li tlalehile ho fokotseha ho tsebahalang ka litlhoko tsa ho lokisa ka ho ntlafatsa bolelele ba li-turing.
4. Khokahano le lik'hemik'hale le li-solven
Kamohelo ea HPLC e nang le lik'hemik'hale tse fapaneng le li-solven li bohlokoa, haholo-holo li-matsalauku tse fapaneng. Liphello tse ling li ka nyenyefatsa boitsebiso ba ho tšosa ka nako e telele, tse eang ho tšoantšetso kapa ho tsoa. Pele u khetha ho khetholla, netefatsa hore o tsamaisana le li-solvents tse sebelisoang ka tatellano ho lab ho thibela litaba tsena.
Mohlala oa Bophelo: Ho lumellana ha mangola a tikoloho
Leeto la liteko tsa tikoloho li etsa tlhahlobo ea pente e fumanoeng e le hore lingoliloeng tsa eona tsa ho ts'oana li ne li sa lumellane le li-solcets tse ling tse sebelisoang ka lisente, tse bakang khafetsa. Ho fetohela ho taring ka mokhoa o lumellaneng ka makhetlo a qobelloang haholo ho fokotsoa haholo le sephetho se ntlafalitsoeng.
5. Ho netefatsa tšusumetso e hloekileng le ea ho se sebetse hantle
Tlhaselo e ka beha liphetho tsa Hplc habonolo habonolo, 'me malinyane e ka ba mohloli o patetsoeng oa makasine ena. Ho hloekisa khafetsa le ho nka sebaka sa bolulo ho thusa kamehla ho boloka botšepehi ba sistimi ea HPpc. Li-Labs tse ngata li kenyelletsa ho fana ka tlhokomelo e reriloeng le ho nkela lipalangoang nako le nako ho qoba hore likotsi tse tlohelang mafu a kang a pharmasical le biochemical.
Theha mokhoa oa ho tsepamisa maikutlo
Ho kenyelletsa tlhahlobo ea kamehla le ho hloekisa li-protocol tsa Hplc ho ka thibela phetoho le phetoho le tšilafalo, e lebisang litholoana tse lumellanang haholoanyane. Li-labs tse ling li sebelisa litsamaiso tsa ho hloekisa kapa tse khethiloeng ho netefatsa hore li tla lula li lokolohile hodima masala.
Khetho e nepahetseng ea li-hipc tubing haholo e ka ntlafatsa haholo ts'ebetso le ho nepahala ha Labs tsa lipatlisiso. Ho tloha ho khetha thepa e loketseng le bophara ba ho laola khatello le ho netefatsa khoebo ea lik'hemik'hale, e 'ngoe le e' ngoe e ama tlhahlobo ea tlhahlobo ea HPLC. Ka ho totobatsa ka hloko lintho tsena, bafuputsi ba ka fihlela ba re ruta lithuto tsa bona le ho kenya letsoho ho pharaletseng saense. Ho lokisa le ho khetha ho lokisa le ho khetha tšebeliso ea litlohetsi feela empa le ho ts'ehetsa boleng ba liphetho tsa lipatlisiso, ho etsa HPTC ho beha karolo ea bohlokoa ho lab.
Nako: Oct-30-2024