Mefuta ea Liquid Chromatography

Ha u rera ho reka lisebelisoa tsa chromatography tsa mokelikeli bakeng sa lipatlisiso tsa laboratori kapa tlhahlobo ea indasteri, u kanna oa khathatsoa ke letoto la lipotso. Ke mofuta ofe oa mokelikeli oa chromatography o loketseng haholo litlhoko tsa hau tsa tlhahlobo, joalo ka ho arola metsoako ea lintho tse phelang tse belang haholo kapa ho sala morao limolek'hule tsa baeloji? U ka etsa bonnete ba hore lisebelisoa tse khethiloeng li kopana le ho nepahala le litlhoko tsa kutlo tsa indasteri ea hau joang? Mme na o utloisisa ka ho hlaka phapang ea maemo a ts'ebeliso lipakeng tsa mefuta e fapaneng ea chromatography ea metsi, e le ho qoba ho se lumellane lipakeng tsa ts'ebetso ea lisebelisoa le mesebetsi ea 'nete ea tlhahlobo?

Chromatography ea metsike theknoloji ea mantlha ea tlhahlobo e sebelisoang haholo mafapheng a kang biopharmaceuticals, polokeho ea lijo le tlhahlobo ea tikoloho. Ho tseba mefuta le litšobotsi tsa eona ho bohlokoa ho khetha lisebelisoa tse nepahetseng. Lintlha tse latelang li tla qaqisa mefuta, mekhahlelo ea lihlahisoa tsa mofuta, melemo, limaraka tsa thepa, le likarolo tsa ts'ebeliso ea liquid chromatography ho u thusa ho etsa liqeto tse nepahetseng.

Mefuta e Tloaelehileng ea Mokelikeli Chromatography

Mmarakeng, chromatography ea metsi e arotsoe haholo ka mefuta e mengata ho latela melao-motheo ea karohano le maemo a ts'ebeliso. High-Performance Liquid Chromatography (HPLC) ke mofuta o sebelisoang ka ho fetesisa, o nang le ts'ebetso e phahameng ea karohano le lebelo la tlhahlobo e potlakileng, e loketseng ho fumanoa ha metsoako e mengata ea manyolo. Ultra-High Performance Liquid Chromatography (UHPLC) e na le khanyetso e phahameng ea khatello le kutlo e betere, e ka khutsufatsang nako ea tlhahlobo ka ho feta 50% ha e bapisoa le HPLC 'me hangata e sebelisoa maemong a liteko tse phahameng. Two-Dimensional Liquid Chromatography (2D-LC) e kopanya mekhoa e 'meli e fapaneng ea karohano, e atolosang mefuta e mengata ea lintho tse lemohuoang, 'me e sebetsa ho hlahloba matrices a rarahaneng a kang serum exogenous exposure. Ntle le moo, ho na le mefuta e ikhethileng e kang Ion-Exchange Chromatography bakeng sa karohano ea metsoako ea ionic le Size-Exclusion Chromatography bakeng sa tlhahlobo ea lintho tse ngata.

Lihlopha tsa Maxi Scientific's Liquid Chromatography

Maxi Scientific Instruments (Suzhou) Co., Ltd., e shebaneng le sebaka sa chromatography, e fana ka mefuta e mengata e felletseng ea lihlahisoa tse amanang le chromatography ea mokelikeli. Lihlahisoa tsa eona tsa mantlha li kenyelletsa Likholomo tsa Ghost-Sniper tse nang le ts'ebetso e ts'oanang le lihlahisoa tsa mantlha, lisebelisoa tsa PEEK HPLC, le li-capillari tsa tšepe tse 316L. Lihlahisoa tsena li na le melemo ea litheko tse theko e tlase, linako tse khutšoane tsa ho fana ka thepa, le ho hanyetsa kutu ho matla. Ka mohlala, capillary ea tšepe e sa hloekang ea 316L e nka mokhoa o khethehileng oa tšireletso, o sa nang le ts'ebetso e babatsehang ea ho tiisa feela empa hape o ka kenngoa habonolo ka letsoho, o loketseng ho lumellana le mekhoa e sa tšoaneng ea chromatography ea metsi.

Monyetla oa Liquid Chromatography

Mabapi le melemo e akaretsang, mokelikeli oa chromatography o ka sekaseka 80% ea metsoako ea lintho tse phelang, haholo-holo tse belang haholo, tse sa tsitsang ka mocheso, le lintho tse sebetsang ka biologically tseo ho leng thata ho li sebetsana le chromatography ea khase. Boikutlo ba eona ba ho lemoha bo phahame, 'me mochine oa ultraviolet o ka fihla ho 0.01ng, o ka finyellang litlhoko tsa tlhahlobo ea trace.

Bakeng sa mefuta e tloaelehileng, HPLC e na le melemo ea litšiea tse ka sebelisoang hape le tšebeliso e nyane ea sampole; UHPLC e na le katleho e ntle ea karohano (ka makhetlo a mararo ho feta ea HPLC e tloaelehileng) le tekanyo e tlaase ea tšilafalo ea sefapano; 2D-LC e ka holisa mefuta e fapaneng ea karohano ea metsi a oli ea lintho tse bonoang ho fihla ho -8 ho isa ho 12, ka ho hlokomela tlhahlobo e phahameng ea lintho tse silafatsang tse ngata.

Lihlahisoa tsa Maxi Scientific li na le melemo e ikhethang. Column ea eona ea Ghost-Sniper e nka mokhoa o ikhethileng oa tlhahiso, o netefatsang ts'ebetso e tsitsitseng ha o ntse o fokotsa litšenyehelo. Lihlahisoa tsa capillary li na le nako e telele ea tšebeletso, e ka fokotsang makhetlo a mangata a ho fetola lisebelisoa bakeng sa basebelisi le ho boloka litšenyehelo tsa ts'ebetso.

Liquid Chromatography Material Grades

Likarolo tsa mantlha tsa chromatography tsa metsi li na le litekanyetso tse thata tsa thepa. Ho nka karolo e le mohlala, mohato o emeng o sebelisa likaroloana tsa porous tse nang le boholo ba 5-10μm bakeng sa HPLC le likaroloana tse nyenyane bakeng sa UHPLC ho ntlafatsa katleho ea karohano. Pipe hangata e entsoe ka tšepe e sa hloekang ea 316L (e hanang ho bola) kapa thepa ea PEEK (e loketseng lisampole tse matla tsa asiti le alkali).

Mabapi le litekanyetso tsa boemo ba indasteri, lisebelisoa li hloka ho finyella matšoao a ts'ebetso a kang ho nepahala ha lebelo la phallo (± 1% kapa ± 2μL / min) le botsitso ba taolo ea mocheso (± 0.1 ℃). Ka mohlala, lihlahisoa tsa Maxi Scientific li lumellana le ISO9001: 2015 setifikeiti sa tsamaiso ea boleng, ho netefatsa hore boleng ba sehlahisoa bo finyella litekanyetso tsa machaba.

Likopo tsa Mokelikeli oa Chromatography

Lefapheng la biopharmaceuticals, chromatography ea metsi e sebelisoa bakeng sa tlhoekiso ea protheine le taolo ea boleng ba lithethefatsi. Ka mohlala, e ka arola le ho lemoha li-amino acid le li-peptide ka lisampole tsa baeloji. Tekong ea polokeho ea lijo, e ka sekaseka li-additives tsa lijo joalo ka li-preservative le litšila tse joalo ka masala a chefo e bolaeang likokoanyana, ka moeli oa ho lemoha o le tlase joalo ka maemo a morao. Tekolong ea tikoloho, e sebelisoa ho bona lintho tse silafatsang tse kang polycyclic aromatics hydrocarbons le phenols metsing le mobung.

Lihlahisoa tsa Maxi Scientific li sebelisitsoe mafapheng a mangata. Morerong oa tlhahlobo ea lijo, Kholo ea eona ea Ghost-Sniper e atlehile ho phethela karohano le ho lemoha lisebelisoa tse ngata tsa lijo, ka sekhahla sa ho hlaphoheloa se fetang 95% le data e tsitsitseng. Morerong oa tlhahlobo ea tikoloho, capillary ea tšepe e sa hloekang ea 316L e tsamaellanang le sistimi ea mokelikeli oa chromatography e hlokometse tlhahlobo e tsoelang pele ea lisampole tsa metsi bakeng sa lihora tse 240, ho netefatsa bonnete ba liphetho tsa liteko.

Qetello

Liquid chromatography e na le mefuta e mengata e kang HPLC, UHPLC, le 2D-LC, e 'ngoe le e' ngoe e na le melemo e ikhethang le maemo a sebetsang. Lihlahisoa tse amanang le chromatography tsa metsi tsa Maxi Scientific, tse nang le ts'ebetso e phahameng, theko e tlase, le boleng bo tšepahalang, li ka fihlela litlhoko tsa liindasteri tse fapaneng. Hore na o etsa lipatlisiso tsa biopharmaceutical, tlhahlobo ea polokeho ea lijo, kapa tlhahlobo ea tikoloho, ho khetha lihlahisoa tsa Maxi Scientific ho ka u thusa ho ntlafatsa katleho ea tlhahlobo le ho fokotsa litšenyehelo. Joale, ka kopo ikopanye le Maxi Scientific hang-hang (call +86 400-6767580) ho fumana litheolelo tsa sehlahisoa le lits'ebeletso tsa tlhahlobo ea pele ho thekiso!