Karolo ea Bohlokoa ea Likopano tsa Lifensetere tsa Flow Cell Optical Window in Liquid Chromatography Diode Array Detection (DAD) Systemscell lens kopano ea fensetere.kopano ea fensetere ea lense ea sele.
Ho Ntlafatsa Khethollo ea Fensetere ea Phallo ea Lisele ho Ntlafatsa Tlhahlobo ea LC le ho Fokotsa Litšenyehelo tsa Tlhokomelo.
Ho k'hemistri ea tlhahlobo moo ho nepahala ho leng bohlokoa haholo, karolo e 'ngoe le e' ngoe ka har'a sistimi ea mokelikeli ea chromatography e kenya letsoho haholo ts'ebetsong. Har'a tsena, kopano ea fensetere ea "flow cell optical" - eo hangata e hlokomolohuoang - e ama ka ho toba ts'ebetso ea phetisetso ea optical, kutloisiso ea mats'oao, le ts'epahalo ea detector ka kakaretso.
Sengoliloeng sena se hlahloba ts'ebetso ea likopano tsa "flow cell optical window", karolo ea tsona ea bohlokoa ho li-diode array detectors (DAD), le hore na khetho e nepahetseng e eketsa nako e telele ea lisebelisoa, e fokotsa nako ea ho sebetsa, le ho ntlafatsa botšepehi ba data.
Phallo Cell Optical Window Assembly: Mosebetsi oa Core
Ha e le hantle, kopano ea fensetere ea "flow cell optical" e sebetsa e le sehokelo sa optical lipakeng tsa mohlala oa phallo le mohloli oa leseli oa detector le sensor array. E thusa phetiso e sa sitisoeng ea leseli la UV-Vis ka leseli la chromatographic, e thusa ho lemoha ka nepo mefuta ea li-analyte ha e ntse e tsoa kholomong ea karohano.
Kopano hangata e na le fensetere ea quartz kapa sapphire optical, likarolo tse shebileng, le ntlo e nepahetseng. Mesebetsi ea eona ea bohlokoa e kenyelletsa:
- Ho boloka tsela e nepahetseng ea optical tsela ka sele e phallang
- Ho thibela ho silafala ha detector optics ka hare
- Ho sireletsa likarolo tse hlokolosi ho senyeha ha lik'hemik'hale le khatello ea maikutlo e bakoang ke khatello
Tšusumetso ho Litekanyetso tsa Ts'ebetso ea DAD
Ho phuputso ea mofuta oa diode - moo leseli la polychromatic ka nako e le 'ngoe le hlokomelang maqhubu a mangata - ho hlaka le ho tsamaisana ho nepahetseng ha ho ka buisanoa. Likopano tsa lifensetere tse sa tloaelehang kapa tse sa lumellaneng li ka baka:
- Karolelano e fokolisitsoeng ea lerata-ho-lerata (SNR)
- Keketseho ea maemo a holimo
- Leseli le khelohileng le phahamisitsoeng le ho nepahala ha wavelength e senyehileng
- Litlhoko tsa ho nchafatsa khafetsa
Likopano tse nang le ts'ebetso e phahameng li netefatsa phetisetso ea leseli e tsitsitseng, e sa sothoeng ka molapo oa sampole, e matlafatsa kutloisiso ea ho lemoha le ho ikatisa ha tlhahlobo-haholo-holo ho bohlokoa bakeng sa tlhahlobo ea morao-rao le ho fumanoa ha motsoako o tlase.
Lintlha tse Bohlokoa tsa Khetho
Khetho e nepahetseng ea kopano ea lifensetere e fetela ka nģ'ane ho ho lumellana ha litekanyo. Etelletsa pele litšobotsi tsena tsa bohlokoa:
1. Maximized Optical Transmission: Optical-grade synthetic quartz e nang le li-anti-reflective coatings e fokotsa tahlehelo ea photon le ho khutlela morao.
2. Tšebelisano ea Lik'hemik'hale: Ho hanana le mefuta e pharalletseng ea pH, li-organic modifiers, le li-buffer system ho thibela etching, deposition, kapa corrosion.
3. Tlhahiso e nepahetseng: Mamello ea sub-micron e netefatsa ho lumellana ka ho sa feleng le ho felisa phapang ea pathlength
4. Moralo o Sebeletsang: Mekhoa ea ho kenya e se nang lisebelisoa e fokotsa nako ea ho lokisa
5. Litekanyetso tse Matla tsa Khatello/Mocheso: Meralo e lumellanang le UHPLC e mamella > 1,500 bar le 90°C maemo a ho sebetsa
Mekhoa e Molemo ka ho Fetisisa ea Ts'ebetso ea Bophelo bo Bolelele
Esita le likopano tse phahameng li hloka tlhokomelo e nepahetseng:
- Etsa tlhahlobo ea pono ea beke le beke bakeng sa li-deposit tse khanyang, mengoallo kapa moholi
- Kenya ts'ebetsong liprothokholo tsa kamora tlhahlobo bakeng sa mekhahlelo ea mohala e nang le letsoai le lengata kapa likaroloana
- Sebelisa torque e boletsoeng ke moetsi nakong ea ho kenya ho thibela ho robeha ha khatello ea kelello
- Tšoara ka liatlana tse se nang lint le lisebelisoa tse ikemiselitseng ho thibela tšoaetso
- Theha lipampiri tsa thibelo ho latela k'hemistri ea mohato oa mobile
Qetello: Precision through Optical Integrity
Tlhahlobong ea sejoale-joale ea chromatographic, botšepehi ba tsela ea optical bo amana ka kotloloho le boleng ba data. Ho tsetela likopanong tsa lifensetere tse entsoeng ka mokhoa o nepahetseng oa "flow cell optical" ho fana ka ROI e lekantsoeng ka mokhoa o matlafalitsoeng oa mekhoa, ho fokotsa maqhubu a ho nchafatsa, le bophelo bo atolositsoeng ba ts'ebeletso ea detector. Ha o ntlafatsa ts'ebetso ea sistimi kapa mathata a ho lemoha mathata, karolo ena ea bohlokoa e hloka tlhahlobo ea ka boomo.
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ChromasirChromasire etsa likopano tsa HPLC tse ntlafalitsoeng tsa lifensetere tse etselitsoeng palo e phahameng ea photon throughput le linako tse atolositsoeng tsa lits'ebeletso. Ikopanye le sehlopha sa rona sa tekheniki ho fana ka tharollo e nepahetseng bakeng sa litlhoko tsa hau tsa tlhahlobo.
Nako ea poso: Jun-05-2025