Ho li-laboratoring tsa hau,Li-chromatograph tsa metsi tse phahameng tsa chromatography (HPLC)Ke mokhoa oa bohlokoa bakeng sa ho arohana, ho tseba le ho khetholla metsoako. Leha ho le joalo, ho fihlela liphetho tse sa fetoheng le tse tšepahalang ha li bolele feela lisebelisoa tse nepahetseng. Ho hlokaKholiseho. Sengoliloeng sena se hlahloba kamoo u ka ntlafatsang ea hauAnalc AnalysisHo eketsa katleho, fokotsa boikemisetso ba ho potlaka, 'me u ntlafatse ho nepahala.
Mathata a tloaelehileng ho tlhahlobo ea HPPT le mokhoa oa ho li rekisa
Ha Hplc ke sesebelisoa se matla sa kemolo, ha se mathata. Litaba tse kangQeto ea ho etsa lintho tse futsanehileng, notlollo ea motheo le liphetho tse sa lumellanenge ka sitisa ts'ebetso ea laboratori. Mona ke mokhoa oa ho sebetsana le mathata ana a tloaelehileng:
1. Qeto e futsanehileng
E 'ngoe ea mathata a tloaelehileng ho HPLC e aroloa hampe pakeng tsa litlhōrō tsa naha, hangata ka lebaka laKhetho ea likopano tse fosahetseng kapa litefiso tsa ho phalla tsa ho phalla. Ho ntlafatsa qeto:
• Khetha aCORromatograkka tse loketsengboholo ba boemo bo phahameng le bo boholobakeng sa litlhahlobo tsa hau.
• Fetolasekhahla sa phallo le maemo a gradientho ntlafatsa bohlanya ba tlhoro le karohano.
• SebelisaTaolo ea mochesoHo tsitsisa linako tsa ho robala le ho ntlafatsa tlhahiso.
2. Ho foka ha motheo kapa lerata
Notloana ea motheo e ka kena-kenana le tlhekefetso ea tlhoro le ho tlohela ho nepahala ha data. Khatiso ena e bakoa ke:
•Mocheso oa mocheso- Boloka tikoloho e tsitsitseng ea laboratoring 'me u sebelise ontong ea kholomo haeba ho hlokahala.
•Karolo ea mobile e silafetseng- Sebelisa li-solvel tse phahameng haholo 'me u behe karolo ea hau ea mobile pele u sebelisoa.
•Tlhaliso ea lisebelisoa- Kamehla hloekisa le ho boloka temole, pompo le malimu ho fokotsa lerata la morao.
3. Khokahano ea Peak ea Peak
Ho hokahanya ho sa lumellaneng ho ama ho tšepahala ha bongata. Ho rarolla sena:
• Netefatsa horeCluck kholomo e na le khotsofalo hantlepele o sebelisoa.
• Boloka aSekhahla sa phallo e tsitsitsengle ho thibela khatello ea khatello.
• ItokisetseLitlhophiso tsa software bakeng sa ho kenella hoa Peak, e netefatsa litholoana tse sa fetoheng le tse hlahisang tlhahiso.
Ho khetha kholomo e nepahetseng ea HPSC
Ho khetha kholomo e nepahetseng ea HPLC keTLHOKOMELISO HO QETELA KOTSI EA HO QETELA. Nahana ka lintlha tsena ha u khetha kholomo:
•Bolelele ba kholomo: Li-Columns tse telele li fana ka karohano e ntle empa e eketsa nako ea likolobe. Khetha bolelele bo lekanyang qeto le lebelo.
•Bophara ba kholomo: Lik'hamera tsa tlholeho li fana ka qeto e phahameng empa e hloka khatello e eketsehileng. Netefatsa ho lumellana le sistimi ea hau ea HPSC.
•Mokhahlelo oa boemo bo phahameng: Khetha mohato ka khekhe e loketseng bakeng sa litlhahlobo tsa hau (mohlala, C18 bakeng sa likhamphani tse seng polaire, Phenyl ea monko o monate oa polar.
Ho lokisa mehala ea mehala ea mehala le litefiso tsa phallo
Mokha oa mobile ke senotlolo ho tlhahlobo ea katleho ea HPTC. Mona ke Tsela ea ho E Fetola:
•Fetola sebopeho sa bohahlauli: Ho itsamaelatekanyo ea soghtho ntlafatsa karohano. SebelisaBoleholimo ba gradientbakeng sa disampole tse thata.
•Laola maemo a ph: NetefatsaPhase Movie Phe lumellana le sampole le kholomo.
•Fokotsa sekhahla sa ho phalla: Litefiso tse phahameng tsa phalle li fokotsa tlhahlobo ea tlhahlobo empa li ka sekisetsa qeto e itseng. Ho leka-lekanya lebelo le katleho e thehiloeng mokhoa oa hau.
Tlhokomelo le Tlhokomelo ea Ts'ebetso
Ho netefatsa tokiso e nepahetsengts'ebetso e sa fetoheng le e nang le sesebelisoa sa bophelo ba lifespan. Latela Mekhoa e metle ka ho fetisisa:
•Mokhoa oa ho hloekisa: Hloekisainjector, kholomo le siptorHo thibela tšilafalo.
•Pheta lits'oants'o: Fetolalitiiso, li-filthara le tubingJoalokaha ho hlokahala ho thibela ho tsoa le ho qobelloa khatello.
•Lekanya sistimi: Likhetho tsa maiketsetso le likarolo tse ling tsa bohlokoa ho netefatsa liphetho tse nepahetseng.
Sephetho
Ho ntlafatsa senakati sa Hplc ho bohlokoa bakeng sa ho ntlafatsa ts'ebetso ea laboratori le ho netefatsa sephetho se phahameng. Ka ho sebetsana le litaba tse tloaelehileng tse kangQeto e futsanehileng, notlo ea motheo le ho kenella ha likhokahano tsa likhoholale ka ho khetha ka nepolikholomo le mehala ea mobile, u ka ntlafatsa haholo ts'ebetso ea hau ea tlhahlobo. KhafetsaTlhokomelo le mokhoa o hlokolosiE tla boloka sistimi ea hau ea HPSC e matha e le katleho ea Peak, e fokotsa nako e nepahetseng, e nyenyefatsa litholoana e nepahetseng, e hlahisoang.
Bakeng sa litsebi li tataisaHplc odmimization, ikopanya leChromasir-Ke ne a ikhethile ho fana kaThabo e hlophisitsoeng ea chromaographyHo thusa laboratori oa hau ho fihlela litekanyetso tse phahameng tsa ts'ebetso.
Nako ea poso: Mar-27-2025