Li laboratori tsa tlhahlobo,High-Performance Liquid Chromatography (HPLC)ke mokhoa oa bohlokoa oa ho arola, ho khetholla le ho lekanya metsoako. Leha ho le joalo, ho fumana liphello tse tsitsitseng le tse ka tšeptjoang ho hloka ho fetang feela lisebelisoa tse nepahetseng-ho hlokahalatokafatso. Sehlooho sena se hlalosa kamoo u ka ntlafatsang boemo ba hau katengTlhahlobo ea HPLCho eketsa ts'ebetso, ho fokotsa nako ea ho theoha, le ho ntlafatsa ho nepahala.
Mathata a Tloaelehileng ho Tlhahlobo ea HPLC le Mokhoa oa ho a Rarolla
Le ha HPLC e le sesebelisoa se matla sa tlhahlobo, ha se na mathata. Litaba tse kangtharollo e fosahetseng, lerata la motheo, le liphetho tse sa tsitsange ka sitisa katleho ea laboratori. Tsela ea ho rarolla mathata ana a tloaelehileng ke ena:
1. Qeto e Fosahetseng
E 'ngoe ea mathata a atileng haholo ho HPLC ke karohano e mpe lipakeng tsa litlhoro, hangata ka lebaka lakhetho e fosahetseng ea kholomo kapa litefiso tse tlase tsa phallo. Ho ntlafatsa tharollo:
• Khetha akholomo ea chromatographicka tse loketsengmohato o emeng le boholo ba karoloanabakeng sa bahlahlobisisi ba hau.
• Fetolasekgahla sa phallo le maemo a kgahlaho matlafatsa sehlohlolong le karohano.
• Sebelisataolo ea mochesoho tsitsisa linako tsa ho boloka le ho ntlafatsa ho ikatisa.
2. Baseline Drift kapa Noise
Lerata la motheo le ka kena-kenana le ho fumanoa ha maemo a holimo le ho senya ho nepahala ha data. Hangata taba ena e bakoa ke:
•Ho fetoha ha mocheso- Boloka tikoloho e tsitsitseng ea laboratori 'me u sebelise onto ea kholomo ha ho hlokahala.
•Mokhahlelo oa mobile o silafetseng- Sebelisa li-solvents tse hloekileng haholo 'me u sefa karolo ea hau ea mohala pele u e sebelisa.
•Tšilafalo ea lisebelisoa- Hloekisa khafetsa 'me u boloke mochine, pompo le li-tubing ho fokotsa lerata le ka morao.
3. Tšebelisano ea Peak e sa tsitsang
Ho kopanya ho sa tsitsang ho ama ho tšepahala ha quantification. Ho rarolla sena:
• Etsa bonnete ba horeKholo ea HPLC e hlophisitsoe hantlepele o sebelisoa.
• Boloka asekhahla sa phallo e tsitsitsengle ho thibela ho fetoha ha khatello.
• Ntlafatsalitlhophiso tsa software bakeng sa kopanyo ea tlhoro, ho netefatsa liphetho tse tsitsitseng le tse ka hlahisoang hape.
Ho Khetha Kholomo e Nepahetseng ea HPLC
Ho khetha kholomo e nepahetseng ea HPLC keya bohlokwa bakeng sa ho fihlella karohano e nepahetseng. Nahana ka lintlha tsena ha u khetha kholomo:
•Bolelele ba Kholomo: Litšiea tse telele li fana ka karohano e ntle empa li eketsa nako ea tlhahlobo. Khetha bolelele bo lekanyang qeto le lebelo.
•Kholomo Diameter: Litšiea tse fokolang li fana ka tharollo e phahameng empa li hloka khatello e eketsehileng. Netefatsa hore e tsamaellana le sistimi ea hau ea HPLC.
•Mokhahlelo oa ho ema: Khetha karolo e nang le k'hemistri e loketseng bakeng sa bahlahlobisisi ba hau (mohlala, C18 bakeng sa metsoako e seng polar, phenyl bakeng sa metsoako e nkhang hamonate).
Ho Ntlafatsa Mekhahlelo ea Mehala le Litefiso tsa Phallo
Karolo ea mohala ke senotlolo sa tlhahlobo e atlehileng ea HPLC. Mona ke mokhoa oa ho e ntlafatsa:
•Fetola sebopeho sa solvent: Hlophisa hantlesolvent ratioho ntlafatsa karohano. Sebelisagradient elutionbakeng sa mehlala e rarahaneng.
•Laola maemo a pH: Etsa bonnete ba horemobile phase pHe lumellana le mohlala le kholomo.
•Ntlafatsa sekhahla sa phallo: Litefiso tse phahameng tsa phallo li fokotsa nako ea tlhahlobo empa li ka senya tharollo. Ho leka-lekanya lebelo le katleho ho latela mokhoa oa hau.
Tlhokomelo le Tlhokomelo ea Thibelo
Tlhokomelo e nepahetseng e tiisatshebetso e tsitsitseng mme e lelefatsa bophelo ba sesebelisoa. Latela mekhoa ena e metle:
•Ho Hloekisa ka Ka mehla: Kamehla hloekisa eaente, kholomo, le detectorho thibela tshilafatso.
•Fetola Lisebelisoa: Phetoholitiiso, li-filters le li-tubingha ho hlokahala ho thibela ho dutla le ho feto-fetoha ha kgatello.
•Lokisa Sisteme: Kamehla lekanya li-detectors le likarolo tse ling tsa bohlokoa ho netefatsa liphetho tse nepahetseng.
Qetello
Ho ntlafatsa tlhahlobo ea HPLC ho bohlokoa bakeng sa ho ntlafatsa katleho ea laboratori le ho netefatsa liphetho tsa boleng bo holimo. Ka ho sebetsana le litaba tse tloaelehileng tse kangtharollo e fosahetseng, lerata la motheo, le ho se lumellane ha tlhōrō ea kopanyo, le ka ho khetha le letonalitšiea le mekhahlelo e tsamaeang, o ka ntlafatsa haholo ts'ebetso ea hau ea tlhahlobo. Ka mehlatlhokomelo le ho ntlafatsa mokhoa o hlokolosie tla boloka sistimi ea hau ea HPLC e sebetsa hantle haholo, e fokotsa nako ea ho theoha le ho netefatsa liphetho tse nepahetseng, tse ka hlahisoang hape.
Bakeng sa tataiso ea litsebi kaHPLC optimization, ho ikopanyaChromasir- re sebetsa ka thata ho fanalitharollo tse ikhethileng tsa chromatographyho thusa laboratori ea hau ho fihlela maemo a holimo a ts'ebetso.
Nako ea poso: Mar-27-2025