Ka har'a mokelikeli o sebetsang hantle haholo oa chromatography (HPLC), likarolo tse 'maloa li bohlokoa haholo kapa li theko e boima joalo ka kholomo ea chromatography. Empa na u ne u tseba hore ka tlhokomelo e nepahetseng le ho sebetsana le eona, u ka eketsa nako ea hau haholonako ea bophelo ea kholomo ea chromatographyle ho ntlafatsa katleho ea lab ea hau ka kakaretso?
Tataiso ena e hlahloba malebela a netefalitsoeng a tlhokomelo le mekhoa e sebetsang e ka u thusang ho sireletsa matsete a hau le ho netefatsa liphetho tse tsitsitseng tsa tlhahlobo ha nako e ntse e ea.
Khetha Mokhahlelo o Nepahetseng oa Mobile ho tloha qalong
Leeto le lelelelenako ea bophelo ea kholomo ea chromatographye qala ka khetho e bohlale ea solvent. Karolo e fosahetseng ea mehala e ka theola thepa ea ho paka, ea fokotsa tharollo, kapa ea baka tšenyo e ke keng ea etsolloa. Kamehla etsa bonnete ba hore pH, matla a ionic, le mofuta oa solvent li lumellana le k'hemistri ea hau e khethehileng.
Ho tlosa lihlapolli le ho li sefa pele li sebelisoa le hona ke mehato ea bohlokoa. Litlhokomelo tsena tse bonolo li thibela ho koaloa ha likaroloana le ho thehoa ha bubble ea khase, tseo ka bobeli li ka senyang ts'ebetso ea kholomo.
Ntlafatsa Mokhoa oa Hao oa Ente
Se kenang kholomong se bohlokoa joalo ka hore na se fihla joang moo. lisampole tse imetsoeng kapa tse nang le li-particulate li ka khutsufatsa ka potlako bophelo bo ka sebelisoang ba kholomo. Sebelisa lisampole tse lokiselitsoeng hantle-tse tlhotliloeng ka li-filters tse 0.22 kapa 0.45 µm-ho thibela ho thibela le khatello ea khatello.
Haeba o sebetsa ka matrices a rarahaneng kapa a litšila, nahana ka ho sebelisa kholomo ea balebeli kapa filthara ea pele ho kholomo. Lisebelisoa tsena tse theko e tlaase li ka tšoasa litšila pele li fihla kholomong ea tlhahlobo, li atoloha haholonako ea bophelo ea kholomo ea chromatography.
Theha Mokhoa oa Kamehla oa ho Hloekisa
Joalo ka lisebelisoa life kapa life tse nepahetseng, kholomo ea hau e hloka ho hloekisoa khafetsa ho boloka ts'ebetso e phahameng. Mokhoa o motle ke ho phunya kholomo ka mor'a ts'ebeliso e 'ngoe le e' ngoe ka solvent e lumellanang, haholo ha o chencha lipakeng tsa sistimi ea buffer kapa mefuta ea sampole.
Ho hloekisa nako le nako ka lihlapolli tse matla ho ka tlosa litšila tse bokeletsoeng le metsoako ea hydrophobic. Etsa bonnete ba hore u latela melaoana e khethehileng ea ho hloekisa 'me u qobe ho sebelisa lik'hemik'hale tse mabifi tse ka senyang karolo ea ho ema.
E boloke Hantle pakeng tsa Runs
Polokelo e nepahetseng hangata e hlokomolohuoa, leha ho le joalo e phetha karolo e kholo ho baballeng ba haunako ea bophelo ea kholomo ea chromatography. Haeba kholomo e ke ke ea sebelisoa nako e telele, e lokela ho hlatsuoa ka mokelikeli o loketseng oa polokelo-hangata o na le motsoako oa lintho tse phelang ho thibela kholo ea likokoana-hloko.
Kamehla koala lipheletsong ka bobeli ka thata ho qoba ho omella kapa ho silafala. Bakeng sa polokelo ea nako e telele, boloka kholomo sebakeng se hloekileng, se laoloang ke mocheso, hole le khanya e tobileng le mocheso.
Lekola Ts'ebetso ea Kholomo Kamehla
Ho boloka lethathamo la khatello ea morao, nako ea ho boloka, le sebopeho sa tlhōrō ho ka u thusa ho tseba matšoao a pele a ho senyeha ha kholomo. Liphetoho tsa tšohanyetso lirapeng tsena li ka bonts'a tšoaetso, voids, kapa frit clogging.
Ka ho tšoara lintlha tsena esale pele, u ka nka khato ea ho lokisa - joalo ka ho hloekisa kapa ho beha sebaka sa balebeli - pele li ama ka ho sa feleng liphetho tsa hau tsa tlhahlobo.
Mehopolo ea ho Qetela
Ho eketsa sebaka sa haunako ea bophelo ea kholomo ea chromatographyha se taba ea ho boloka chelete feela - ke ho boloka ts'epo ea data, ho fokotsa nako ea ho fokotsa nako, le ho ntlafatsa tlhahiso ea lab. Ka leano le nepahetseng la tlhokomelo ea thibelo, o ka sireletsa e 'ngoe ea thepa ea hau ea bohlokoa ka ho fetisisa ea lab mme oa netefatsa liphetho tse tšepahalang haholoanyane nakong e ngoe le e ngoe.
Na u hloka likeletso tsa litsebi mabapi le mekhoa ea chromatographic kapa khetho ea sehlahisoa?IkopanyeChromasirkajeno-re mona ho ts'ehetsa katleho ea lab ea hau ka temohisiso ea tekheniki le litharollo tse ikhethileng.
Nako ea poso: Apr-11-2025