Ho boloka kholomo ea hau ea chromatography e le maemong a nepahetseng ha se mokhoa o motle feela - ho bohlokoa bakeng sa liphetho tse nepahetseng le ts'ebeliso ea nako e telele ea litšenyehelo. Hore na o sebetsa tlhahlobong ea meriana, polokeho ea lijo, kapa tlhahlobo ea tikoloho, ho ithuta ho lelefatsa bophelo ba kholomo ea hau ea chromatography ho tla fokotsa nako, ho ntlafatsa ho ikatisa, le ho thusa ho boloka ts'ebetso e tsitsitseng.
Polokelo e Loketseng e Etsa Phapang Tsohle
E 'ngoe ea lintlha tse hlokomolohuoang ka ho fetesisa tsa tlhokomelo ea likholomo ke polokelo e nepahetseng. Maemo a sa nepahalang a polokelo a ka lebisa kholong ea likokoana-hloko, mouoane oa solvent, le tšenyo e ke keng ea etsolloa. Kamehla latela litataiso tse nepahetseng tsa polokelo ho latela mofuta oa kholomo ea chromatography eo u e sebelisang. Ka mohlala, ha u boloka litšiea tse khutliselitsoeng nako e telele, hlatsoa ka motsoako o nang le bonyane 50% ea motsoako oa tlhaho, 'me u tiise lipheletsong tse peli ka thata. Haeba u sebelisa li-mobile phases tse sirelelitsoeng, qoba ho tlohella sekoaelo hore se omelle ka har'a kholomo, kaha sena se ka baka pula ea letsoai le ho thiba.
Ho Thibela ho Koaleha le Tšilafalo
Ho qoba ho silafala ke e 'ngoe ea litsela tse sebetsang ka ho fetisisa tsa ho lelefatsa bophelo ba likholomo. Ho hlophisoa ha mekhahlelo ea mehala le lisampole ho bohlokoa. Sebelisa lifilthara tsa 0.22 µm kapa 0.45 µm ho tlosa likaroloana pele ho ente. Ho feta moo, ho nkela litiiso tse tsofetseng khafetsa, lisyringe le linkho tsa sampole li netefatsa hore ha ho na lintho tse tsoang kantle ho naha tse kenang tsamaisong. Bakeng sa lilaboratori tse sebelisang matrices a rarahaneng kapa a litšila, kholomo ea balebeli e ka sebetsa e le mokhoa oa pele oa tšireletso khahlanong le litšila tse amanang le sampole-ho monya litšila pele li fihla kholomong ea tlhahlobo.
Ho Hlatsoa le ho Hloekisa ka Ka mehla ha ho ka Buisanoa
Haeba kholomo ea hau ea chromatography e sebelisoa khafetsa, ho hloekisa kamehla ho bohlokoa. Ho hloekisa nako le nako ho tlosa metsoako e setseng e ka bakang lerata la motheo, litlhōrō tsa ghost, kapa tahlehelo ea tharollo. Hlakola kholomo ka solvent e tsamaellanang le mobile phase empa e le matla ho lekana ho hlatsoa thepa efe kapa efe e bolokiloeng. Bakeng sa litšiea tse khutliselitsoeng morao, motsoako oa metsi, methanol, kapa acetonitrile o sebetsa hantle. Kenyelletsa kemiso ea ho hloekisa beke le beke ho latela makhetlo le mofuta oa litlhahlobo tse etsoang ho thibela ho aha le ho netefatsa katleho e phahameng.
Sebelisa Lisefe tsa Pele ho Kholomo le Likholomo tsa Balebeli
Ho kenya filthara ea pre-column kapa kholomo ea balebeli ke letsete le lenyenyane le nang le phaello e kholo. Likaroloana tsena li nka li-particulate le metsoako e bolokiloeng ka matla pele li ka kena kholomong ea mantlha ea tlhahlobo. Ha li lelefatse bophelo ba kholumo ea hau ea chromatography feela empa hape li e sireletsa ho tsoa khatellong ea tšohanyetso e bakoang ke litšitiso. Le ha lisebelisoa tsena li hloka ho nkeloa sebaka nako le nako, li theko e tlase haholo ho feta ho nkela kholomo e felletseng ea tlhahlobo.
Malebela a Tlhokomelo bakeng sa Basebelisi ba HPLC
Bakeng sa basebelisi ba HPLC, ho ela hloko khatello ea tsamaiso le litekanyetso tsa phallo li ka fana ka matšoao a pele a ho senyeha ha kholomo. Keketseho ea tšohanyetso ea khatello ea mokokotlo hangata e bontša ho koala, ha linako tse ntseng li hoholeha li ka fana ka maikutlo a ho thiba ho itseng kapa ho fokotseha ha karolo. Ho sebelisa litekanyo tse nepahetseng tsa phallo le ho qoba liphetoho tse matla tsa khatello ho tla sireletsa botšepehi ba ho paka litšiea le karolo ea eona e emeng. Ho feta moo, qoba ho pepesa kholomo ho lihlapolli tse sa lumellaneng kapa maemo a pH ka ntle ho sebaka sa eona se khothalletsoang, kaha tsena li ka baka ho senyeha ka potlako.
Mehopolo ea ho Qetela
Kholomo ea hau ea chromatography ke karolo ea bohlokoa ea sistimi ea hau ea tlhahlobo, 'me ka tlhokomelo e nepahetseng, e ka fana ka likete tsa liente tsa boleng bo holimo. Ho tloha polokelong e nepahetseng ho isa ho ho hloekisa le ho sefa ka matla, ho amohela mohopolo oa pele oa tlhokomelo ha o boloke boleng ba data feela empa hape o fokotsa litšenyehelo tsa ho khutlisa.
U batla ho ntlafatsa tšebetso ea lab ea hau ea chromatography? Fumana litharollo tse ka tšeptjoang le tataiso ea litsebi hoChromasir-moo ho nepahala ho kopanang le ho tšepahala. Re u thuse ho lelefatsa bophelo ba lisebelisoa tsa hau le ho phahamisa liphetho tsa hau.
Nako ea poso: Apr-23-2025