Chromatography ke mokhoa oa bohlokoa haholo lipatlisisong tsa morao-rao tsa saense, empa ho hlaha halitlhōrō tsa moeaka chromatogram e ka hlahisa mathata a bohlokoa ho bahlahlobisisi. Litlhōrō tsena tse sa lebelloang, tse atisang ho hlaha nakong ea karohano ea chromatographic, haholo-holo ka mokhoa oa gradient, li senya tlhahlobo ea palo le ho senya nako ea bohlokoa. Ho utloisisa lisosa tsa litlhoro tsa ghost le ho sebelisa litharollo tse ncha tse kang tsa ChromasirSehlopha sa Ghost-Snipere ka fetola mekhoa ea hau ea tlhahlobo.
Ghost Peaks ke Eng 'me Ke Hobane'ng ha e Le Bohlokoa?
Litlhōrō tsa Ghost ke matšoao a sa tsejoeng a chromatogram a sitisang ho hlaka ha liphetho tsa tlhahlobo. Le hoja li ka 'na tsa bonahala li le nyenyane, phello ea tsona e ka ba ea bohlokoa:
1. Mathata a mangata
Ha litlhoro tsa ghost li kopana le tlhoro ea thahasello, li thatafatsa palo e nepahetseng ea bahlahlobisisi. Sena se ka lebisa ho litlhaloso tse fosahetseng tsa data le liphetho tse sa tšepahaleng.
2. Ho rarolla mathata ka nako
Ho hlwaya mohloli oa litlhoro tsa lithotsela hangata ho hloka lipatlisiso tse telele, ho faposa maikutlo a bahlahlobisisi mesebetsing ea bohlokoa. Nako e sebelisoang ho rarolla mathata ana e ka ntlafatsa tlhahiso le liphetho tsa lipatlisiso.
Ghost Peaks e Tsoa Hokae?
Ho felisa litlhoro tsa ghost ka katleho, ho bohlokoa ho utloisisa tšimoloho ea bona. Litlhoro tsena tse sa lebelloang hangata li hlaha ho tsoa ho litšila ho:
1.Likarolo tsa Sisteme:Masalla a tsamaiso ea chromatographic a ka kenya letsoho ho litlhōrō tsa ghost.
2.Likholomo:Litšila tse ka har'a thepa ea ho paka kapa ho senyeha ha tšebeliso li ka baka tšilafalo.
3.Mehlala:Mehlala e silafetseng e hlahisa metsoako e sa lebelloang ka har'a chromatogram.
4.Mohato oa Mobile:Litšila tse tsoang ho lihlapolli, letsoai la buffer, kapa likarolo tse nang le metsi / manyolo hangata li kenya letsoho tlhorong ea lipoko.
5.Lijana:Mehlala ea libotlolo tsa ho lokisa le lijana tse ling li ka hlahisa litšila tse setseng.
Tharollo ea Phetoho: The Ghost-Sniper Column
Chromasir'sSehlopha sa Ghost-Sniperke tharollo e fetolang papali e etselitsoeng ho loantša litlhoro tsa ghost. Karolo ena ea moloko oa bobeli e na le sebopeho se ntlafalitsoeng le lisebelisoa tse tsoetseng pele tsa ho paka, tse e nolofalletsang ho hapa litlhōrō tsa ghost esita le tlas'a maemo a feteletseng. Ho sebetsa ha eona ho etsa hore e be sesebelisoa sa bohlokoa bakeng sa ho netefatsa mekhoa le ho hlahloba mohlala.
Lilaboratori tse sebelisang Kholo ea Ghost-Sniper li tlalehile lintlafatso tse kholo tsa boleng ba chromatogram, linako tse fokolitsoeng tsa ho rarolla mathata, le tlhahiso e ntlafalitsoeng ka kakaretso.
Mokhoa oa ho eketsa Melemo ea Likholomo tsa Ghost-Sniper
Bakeng sa ts'ebetso e nepahetseng, latela mehato ena ea tlhokomelo le mekhoa e metle:
1.Tekanyo ea Nako ea Tekanyo:
Kenya metsotso e 5–10 ea nako e leka-lekaneng litsamaisong tsa HPLC ho amohela bophahamo ba kholomo.
2.Tlhophiso ea pele:
Hlakola litšiea tse ncha ka 100% acetonitrile ka tekanyo ea phallo ea 0.5 mL / min bakeng sa lihora tsa 4 pele o sebelisoa ho netefatsa ts'ebetso e hloekileng le e sebetsang.
3.Tšoara li-reagents tsa Ion-Pair ka hloko:
Li-reagents tsa ion-pair tse karolong ea mobile li ka fetola linako tsa ho boloka le libopeho tse phahameng. Sebelisa ka hloko ha li-reagents tse joalo li le teng.
4.Fetola Likholomo Hangata:
Nako ea bophelo ea kholumo e ipapisitse le lintlha tse kang bohloeki ba karolo ea mobile, maemo a solvent, le bohloeki ba lisebelisoa. Ho fetola kamehla ho netefatsa liphetho tse tsitsitseng.
5.Mehato ea Flush e nang le Letsoai:
Sebelisa 10% ea tharollo ea organic phase (mohlala, methanol kapa acetonitrile) pele le ka mor'a ho sebelisa mekhahlelo e tsamaeang e nang le letsoai ho thibela ho thibela.
6.Boloka ka nepo nakong ea Phomolo:
Bakeng sa polokelo ea nako e telele, boloka kholomo ka har'a 70% ea organic aqueous solution (methanol kapa acetonitrile). Hlakola ka 100% acetonitrile pele u e sebelisa hape ho khutlisetsa ts'ebetso.
7.Lekola Ts'ebetso:
Fetola kholumo haeba phello ea eona ea ho hapa e fokotseha kapa haeba litlhoko tsa tlhahlobo li feta bokhoni ba eona.
Hobaneng Likholomo tsa Ghost-Sniper li le Bohlokoa bakeng sa Lab ea Hau
The Ghost-Sniper Column ha se sesebelisoa sa ho rarolla mathata; e ntlafatsa ts'ebetso ea kakaretso ea sistimi ea hau ea chromatographic.
•E Felisa Ghost Peaks:Esita le maemong a thata ka ho fetisisa, karolo ena e hapa litlhōrō tsa lithotsela ka katleho.
•E Sireletsa Thepa:E sefa likaroloana tse tiileng le lintho tse silafatsang tse phelang, e sireletsang lisebelisoa le litšiea tsa chromatographic.
•E Ntlafatsa Boleng ba Boitsebiso:Ka ho felisa litšitiso, kholomo e hlahisa li-chromatogram tse hloekileng, tse tšepahalang haholoanyane.
Lisebelisoa tsa Maxi Scientific: Molekane oa Hao ka Bokhabane ba Tlhahlobo
At Maxi Scientific Instruments (Suzhou) Co., Ltd., re fana ka litharollo tse ncha tsa ho matlafatsa lilaboratori lefatšeng ka bophara. Likholomo tsa rona tsa Ghost-Sniper li entsoe ka makhethe ho sebetsana le liphephetso tsa litlhoro tsa ghost, ho netefatsa liphetho tse nepahetseng le phallo e bonolo ea mosebetsi.
Ntlafatsa Chromatography ea hau Kajeno
Se ke oa lumella litlhoro tsa ghost ho sitisa lipatlisiso tsa hau. Tsetela ho Likholomo tsa Chromasir's Ghost-Sniper 'me u bone phapang ea ho nepahala, ho sebetsa hantle le khotso ea kelello. IkopanyeMaxi Scientific Instruments (Suzhou) Co., Ltd.kajeno ho ithuta haholoanyane mabapi le hore na litharollo tsa rona li ka phahamisa tšebetso ea laboratori ea hau joang. Hammoho, ha re fihleleng bokhabane ho chromatogram e ngoe le e ngoe.
Nako ea poso: Dec-13-2024