Kholomo ea Ghost-Sniper ea Chromasir HPLC UPLC e tlosa litlhoro tsa ghost

Ghost-Sniper Column Chromasir HPLC UPLC kholomo e tlosa litlhoro tsa ghost Setšoantšo se bontšitsoeng

tlhaloso e khuts'oane:

Kholomo ea Ghost-Sniper ke sesebelisoa se matla sa ho felisa litlhoro tsa ghost tse hlahisoang nakong ea karohano ea chromatographic, haholo-holo ka mokhoa oa gradient. Litlhōrō tsa ghost li tla baka mathata a mangata haeba litlhōrō tsa ghost li feta litlhōrō tsa thahasello. Ka kholomo ea Chromasir ghost-sniper, liqholotso tsohle tsa litlhoro tsa ghost li ka rarolloa mme litšenyehelo tsa tšebeliso ea liteko li ka theoha haholo.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Kholomo ea Ghost-sniper e entsoe ka ho khetheha ho felisa litlhoro tsa ghost. Litlhōrō tsa Ghost ke tsa tšimoloho e sa tsejoeng ho chromatogram, hangata e bonoa nakong ea karohano ea chromatographic ho gradient elution kapa ts'ebetso ea nako e telele. Ho hlaha ha litlhōrō tsa moea ho na le tšusumetso e tebileng litekong tsa bahlahlobisisi. Kholomo ea Ghost-sniper e etsahala ho rarolla mathata ao le ho etsa hore ts'ebetso ea liteko e be bonolo le ho sebetsa hantle. Kholomo ena e ka sebelisoa maemong a feteletseng mme e bonts'a phello e kholo ea ho hapa. Ke mokhoa o motle oa ho tlosa tšitiso ea litlhoro tsa ghost mabapi le netefatso ea mekhoa le ho latela tlhahlobo ea lintho.

Mekhahlelo

Karolo No.	Boemo	Bolumo	Kopo
MC5046091P	50 × 4.6 limilimithara	hoo e ka bang 800ul	HPLC
MC3546092P	35 × 4.6 limilimithara	hoo e ka bang 580ul	HPLC
MC5021093P	50 × 2.1 limilimithara	hoo e ka bang 170ul	UPLC
MC3040096P	30 × 4.0 limilimithara	hoo e ka bang 380ul	HPLC Bophahamo bo tlase ba kholomo

Ho kenya

Kopo le Liphetho

Mehato ya tlhokomelo

1. Haeba tlhahlobo ea batch e hlophisitsoe tsamaisong ea HPLC, ho kgothaletswa ho eketsa nako e eketsehileng ea ho leka-lekana bakeng sa metsotso e ka bang 5 - 10 min maemong a hau a chromatographic, bakeng sa tšusumetso ea boholo ba kholomo ea ghost-sniper.
2. Bakeng sa litšiea tse ncha, li hlatsoe ka 100% acetonitrile ka tekanyo ea phallo ea 0.5ml / min bakeng sa lihora tse 4 pele o sebelisoa.
3. Li-reagents tsa Ion-pair tse tsamaeang, li ka 'na tsa nkoa ke ghost-sniper column, e ka susumetsang nako ea ho boloka le sebopeho sa tlhōrō ea sepheo sa hau. Ka kopo, sebelisa ka hloko maemong a joalo a mobile.
4. Nako ea bophelo ea kholomo e itšetlehile ka maemo a tlhahlobo, joalo ka karolo ea mobile. ho hloeka ha solvent, le ho silafatsoa ha thepa. Ka kopo, khutlisa kholomo ea ghost-sniper khafetsa ho netefatsa ts'ebetso.
5. Ho fetola kholomo ea ghost-sniper hoa khothaletsoa haeba phello ea ho hapa e mpefala kapa litlhoko li sa khotsofatsoe.
6. Joalo ka karolo ea tlhoekiso ho chromatograph ea mokelikeli, kholomo ea ghost-sniper e ka sefa likaroloana tse tiileng le ho tlosa litšila tsa lintho tse phelang pele ho ente. Kholomo ea ghost-sniper e boetse e fana ka lisebelisoa le kholomo ea chromatographic ka ts'ireletso e betere, hape e ntlafatsa chromatogram.
7. Haeba mokhahlelo o tsamaeang o na le letsoai pele le ka mor'a ho le sebelisa, le fetisetse ho metsi ka 10% ea motsoako oa organic phase (10% methanol kapa acetonitrile), ho qoba letsoai le thibelang ho tsoa le ho thibela kholomo.
8. Ka kopo hlokomela hore ha se litlhōrō tsohle tsa ghost tse ka haptjoang ke kholomo ea Ghost-Sniper.
9. Haeba kholomo e siea e sa sebelisoe ka nako e telele, ho kgothaletswa ho e boloka ka har'a tharollo ea metsi a manyolo (70% methanol kapa acetonitrile). 'Me ka kopo e hlatsoe ka 100% acetonitrile ka tekanyo ea phallo ea 0.5 ml / min bakeng sa hora ea 1 pele u sebelisa.